Prof. Teresa Carlomagno | Carlomagno Group

Academic Director of Biomolecular NMR

Professor of Structural Biology

t.carlomagno@bham.ac.uk

Main office: Room 807, Biosciences
NMR office: Room 104, HWB-NMR

More info:

University of Birmingham Profile

University of Birmingham Research Portal

Affiliation(s)

School of Biosciences, College of Life and Environmental Sciences

School of Medical Sciences, College of Medicine and Health

About

Teresa Carlomagno started her scientific career at the University of Naples, Italy, where she studied Chemistry. During her Ph.D. studies and her following post-doctoral work at the University of Frankfurt, Germany, she developed NMR methods to study the structure and the dynamics of proteins, nucleic acids and small molecules. During a second post-doctoral period at The Scripps Research Institute, La Jolla, USA, she matured a strong interest in the mechanisms of intermolecular recognition and function in ribonucleoprotein complexes.

In 2001, she joined the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany, and started her own research group. In 2007, she moved to the EMBL in Heidelberg as a group leader in biomolecular NMR spectroscopy in the Structural and Computational Biology Unit. In 2015 she moved to the Leibniz University of Hannover, as a W3 professor in Structural Chemistry and joined group leader at the HZI in Braunschweig. In 2021 she left Germany to continue her career at the University of Birmingham, where she is Professor of Integrative Structural Biology at the School of Biosciences and in the Institute of Cancer and Genomics Sciences, as well as Academic Director of the national high-field NMR facility HWB-NMR.

Her laboratory characterizes intermolecular interactions at the border between structure, dynamics and disorder by combining NMR spectroscopy both in solution and in solid-state with XRS, EM, Small-Angle-Scattering (SAS) and Electron Paramagnetic Resonance (EPR). She uses integrative structural biology to study macromolecular complexes with enzymatic function involved in RNA metabolism and in the regulation of gene expression. In parallel, the lab develops the computational methodologies necessary to interpret and evaluate integrative structural biology data and new methodologies for structural biology of RNA. The lab has pioneered solid-state NMR as a tool to solve structures of RNA in flexible RNP complexes, which are not suitable for XRS and EM studies.

Awards and Honours:

Member of the DFG Committee on Scientific Instrumentation and Information Technology 2017–2022
Chair of the ENC (conference with ca. 1000 participants) in Asilomar, California – April 19–24, 2015
Chair of the EMBO/EMBL Symposium "Molecular Machines: Lessons from integrating structure, biophysics and chemistry" – Heidelberg, Germany – May 18–21, 2014
Richard Ernst Stipendium from the Group of Magnetic Resonance of the GdCH (Gesellschaft Deutscher Chemiker, German Chemical Society) 1999
Alexander von Humboldt fellowship, 1997 (award declined in favor of the Marie Curie fellowship)
European Union grant of the "Marie Curie Researcher Training and Mobility Program", 1997
Grant from the Italian Ministry of University, 1995
EMBO (European Molecular Biology Organization) fellowship, 1995

Publications within the group:

Long-range conformational changes in the nucleotide-bound states of the DEAD-box helicase Vasa	Biophysical Journal	2024
The specificity of intermodular recognition in a prototypical nonribosomal peptide synthetase depends on an adaptor domain	Science Advances	2024
Phosphopeptide binding to the N-SH2 domain of tyrosine phosphatase SHP2 correlates with the unzipping of its central β-sheet	Computational and Structural Biology Journal	2024
Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR	Methods in Molecular Biology	2023
NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes	Methods in Molecular Biology	2023
An NMR Study of a 300-kDa AAA+ Unfoldase	Journal of Molecular Biology	2023
Experiment-guided molecular simulations define a heterogeneous structural ensemble for the PTPN11 tandem SH2 domains	Chemical Science	2023
A real-time analysis of GFP unfolding by the AAA+ unfoldase PAN	Journal of Magnetic Resonance	2023
The EJC disassembly factor PYM is an intrinsically disordered protein and forms a fuzzy complex with RNA	Frontiers in Molecular Biosciences	2023
Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2	Structure	2023
Nucleic acid–protein interfaces studied by MAS solid-state NMR spectroscopy	Journal of Structural Biology: X	2022
Identification of RNA Base Pairs and Complete Assignment of Nucleobase Resonances by Proton-Detected Solid-State NMR Spectroscopy at 100 kHz MAS	Angewandte Chemie International Edition	2021
1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein)	Biomolecular NMR Assignments	2021
Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites	Scientific Reports	2021
Recruitment of phospholipase Cγ1 to the non-structural membrane protein pK15 of Kaposi Sarcoma-associated herpesvirus promotes its Src-dependent phosphorylation	PLOS Pathogens	2021
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications	Frontiers in Molecular Biosciences	2021
Phosphotyrosine couples peptide binding and SHP2 activation via a dynamic allosteric network	Computational and Structural Biology Journal	2021
High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain	RNA	2021
Structural and functional roles of 2′-O-ribose methylations and their enzymatic machinery across multiple classes of RNAs	Current Opinion in Structural Biology	2020
1H, 13C, 15N chemical shift assignments of SHP2 SH2 domains in complex with PD-1 immune-tyrosine motifs	Biomolecular NMR Assignments	2020
Observing Protein Degradation by the PAN-20S Proteasome by Time-Resolved Neutron Scattering	Biophysical Journal	2020
Specificity and regulation of phosphotyrosine signaling through SH2 domains	Journal of Structural Biology: X	2020
Structure of a Protein–RNA Complex by Solid-State NMR Spectroscopy	Angewandte Chemie International Edition	2020
The guide sRNA sequence determines the activity level of box C/D RNPs	eLife	2020
Small-Angle Neutron Scattering of RNA–Protein Complexes	Methods in Molecular Biology	2020
Molecular mechanism of SHP2 activation by PD-1 stimulation	Science Advances	2020
Molecular mechanism of SHP2 activation by PD-1 stimulation	Science Advances	2020
Histone chaperone exploits intrinsic disorder to switch acetylation specificity	Nature Communications	2019
Solid-state NMR Spectroscopy of RNA	Methods in Enzymology	2018
Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS	Chemical Communications	2018
Isotope labeling for studying RNA by solid-state NMR spectroscopy	Journal of Biomolecular NMR	2018
Structural characterization of the Asf1–Rtt109 interaction and its role in histone acetylation	Nucleic Acids Research	2018
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth	Origins of Life and Evolution of Biospheres	2017
M3: an integrative framework for structure determination of molecular machines	Nature Methods	2017
Structure-Based Design of Scaffolds Targeting PDE10A by INPHARMA-NMR	Journal of Chemical Information and Modeling	2017
A bromodomain–DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT	Nature Communications	2016
RflM mediates target specificity of the RcsCDB phosphorelay system for transcriptional repression of flagellar synthesis in Salmonella enterica	Molecular Microbiology	2016
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth Part I: Chemical Diversity, Oxygen and Nitrogen Based Polymers	Origins of Life and Evolution of Biospheres	2016
Archaea box C/D enzymes methylate two distinct substrate rRNA sequences with different efficiency	RNA	2016
The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats	Nucleic Acids Research	2016
Optimization of protein samples for NMR using thermal shift assays	Journal of Biomolecular NMR	2016
On-the-Fly Integration of Data from a Spin-Diffusion-Based NMR Experiment into Protein–Ligand Docking	Journal of Chemical Information and Modeling	2015
A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin	Structure	2015
The structure of the SOLE element of oskar mRNA	RNA	2015
Identification of new hit scaffolds by INPHARMA-guided virtual screening	MedChemComm	2015
RNA structure determination by solid-state NMR spectroscopy	Nature Communications	2015
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum	Nucleic Acids Research	2014
Six Subgroups and Extensive Recent Duplications Characterize the Evolution of the Eukaryotic Tubulin Protein Family	Genome Biology and Evolution	2014
Molecular Recognition of Epothilones by Microtubules and Tubulin Dimers Revealed by Biochemical and NMR Approaches	ACS Chemical Biology	2014
Present and future of NMR for RNA–protein complexes: A perspective of integrated structural biology	Journal of Magnetic Resonance	2014
The structure of the box C/D enzyme reveals regulation of RNA methylation	Nature	2013
A Reducing Milieu Renders Cofilin Insensitive to Phosphatidylinositol 4,5-Bisphosphate (PIP2) Inhibition	Journal of Biological Chemistry	2013
A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex	Angewandte Chemie International Edition	2013
Accounting for Conformational Variability in Protein–Ligand Docking with NMR-Guided Rescoring	Journal of the American Chemical Society	2013
Structural Principles of RNA Catalysis in a 2′–5′ Lariat-Forming Ribozyme	Journal of the American Chemical Society	2013
Towards Improved Quality of GPCR Models by Usage of Multiple Templates and Profile-Profile Comparison	PLOS ONE	2013
Protein–RNA Interfaces Probed by 1H-Detected MAS Solid-State NMR Spectroscopy	Angewandte Chemie International Edition	2013
Synthesis and biological evaluation of a water-soluble derivative of the potent V-ATPase inhibitor archazolid	Bioorganic & Medicinal Chemistry Letters	2012
Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity	Nature Structural and Molecular Biology	2012
The description of protein internal motions aids selection of ligand binding poses by the INPHARMA method	Journal of Biomolecular NMR	2012
Understanding the Inhibitory Effect of Highly Potent and Selective Archazolides Binding to the Vacuolar ATPase	Journal of Chemical Information and Modeling	2012
Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding	Angewandte Chemie International Edition	2012
NMR in natural products: understanding conformation, configuration and receptor interactions	Natural Product Reports	2012
An NMR-based scoring function improves the accuracy of binding pose predictions by docking by two orders of magnitude	Journal of Biomolecular NMR	2012
Computational techniques are valuable tools for the discovery of protein–protein interaction inhibitors: The 14-3-3σ case	Bioorganic & Medicinal Chemistry Letters	2011
Recognition of 2′-O-Methylated 3′-End of piRNA by the PAZ Domain of a Piwi Protein	Structure	2011
Interaction of Epothilone B (Patupilone) with Microtubules as Detected by Two-Dimensional Solid-State NMR Spectroscopy	Angewandte Chemie International Edition	2010
Structure of the K-turn U4 RNA: a combined NMR and SANS study	Nucleic Acids Research	2010
Peptide Ligands Selected with CD4-Induced Epitopes on Native Dualtropic HIV-1 Envelope Proteins Mimic Extracellular Coreceptor Domains and Bind to HIV-1 gp120 Independently of Coreceptor Usage	Journal of Virology	2010
The Tubulin-Bound Structure of the Antimitotic Drug Tubulysin	Angewandte Chemie International Edition	2010
Synthesis and Biological Characterization of Argyrin F	ChemMedChem	2010
The Binding Mode of Side Chain- and C3-Modified Epothilones to Tubulin	ChemMedChem	2010
Elucidation of the Structure and Intermolecular Interactions of a Reversible Cyclic-Peptide Inhibitor of the Proteasome by NMR Spectroscopy and Molecular Modeling	Angewandte Chemie International Edition	2010
1H, 13C and 15N resonance assignments of the Calmodulin-Munc13-1 peptide complex	Biomolecular NMR Assignments	2010
Intermolecular Protein−RNA Interactions Revealed by 2D 31P−15N Magic Angle Spinning Solid-State NMR Spectroscopy	Journal of the American Chemical Society	2010
Drug Design for G-Protein-Coupled Receptors by a Ligand-Based NMR Method	Angewandte Chemie International Edition	2010
Modular architecture of Munc13/calmodulin complexes: dual regulation by Ca2+ and possible function in short-term synaptic plasticity	EMBO Journal	2010
The INPHARMA technique for pharmacophore mapping: A theoretical guide to the method	Journal of Magnetic Resonance	2009
An Efficient Strategy for the Determination of the Three-Dimensional Architecture of Ribonucleoprotein Complexes by the Combination of a Few Easily Accessible NMR and Biochemical Data: Intermolecular Recognition in a U4 Spliceosomal Complex	Journal of Molecular Biology	2009
Probing Mutation-Induced Structural Perturbations by Refinement Against Residual Dipolar Couplings: Application to the U4 Spliceosomal RNP Complex	ChemBioChem	2009
NMR assignments of HIV-2 TAR RNA	Biomolecular NMR Assignments	2008
Crystallography-Independent Determination of Ligand Binding Modes	Angewandte Chemie International Edition	2008
Specific Methyl Group Protonation for the Measurement of Pharmacophore-Specific Interligand NOE Interactions	Chemistry: A European Journal	2008
Studies of dynamic nuclear polarization with nitroxides in aqueous solution	Applied Magnetic Resonance	2008
Construction of a liquid-state NMR DNP shuttle spectrometer: First experimental results and evaluation of optimal performance characteristics	Applied Magnetic Resonance	2008
Argyrin A Reveals a Critical Role for the Tumor Suppressor Protein p27kip1 in Mediating Antitumor Activities in Response to Proteasome Inhibition	Cancer Cell	2008
Simultaneous Determination of the Conformation and Relative Configuration of Archazolide A by Using Nuclear Overhauser Effects, J Couplings, and Residual Dipolar Couplings	Angewandte Chemie International Edition	2008
Field dependent dynamic nuclear polarization with radicals in aqueous solution	Journal of the American Chemical Society	2008
Conformational preferences of natural and C3-modified epothilones in aqueous solution	Journal of Medicinal Chemistry	2008
13C-Detection in RNA Bases: Revealing Structure−Chemical Shift Relationships	Journal of the American Chemical Society	2007
Binding of the Human Prp31 Nop Domain to a Composite RNA–Protein Platform in U4 snRNP	Science	2007
High-Resolution Solid-State NMR Structure of an Anticancer Agent	ChemMedChem	2007
Structural Basis of the Activity of the Microtubule-Stabilizing Agent Epothilone A Studied by NMR Spectroscopy in Solution	Angewandte Chemie International Edition	2007
The Tubulin-Bound Conformation of Discodermolide Derived by NMR Studies in Solution Supports a Common Pharmacophore Model for Epothilone and Discodermolide	Angewandte Chemie International Edition	2006
Calculation of the Dependence of Homo- and Heteronuclear 3J and 2J Scalar Couplings for the Determination of the 2′-Hydroxy Conformation in RNA	Angewandte Chemie International Edition	2006
Stereochemical Determination of Archazolid A and B, Highly Potent Vacuolar-Type ATPase Inhibitors from the Myxobacterium Archangium gephyra	Organic Letters	2006
SHARP-TACSY: Triple-Band Tailored Correlated Spectroscopy for Base-to-Sugar Transfer in Nucleic Acid Residues with Intermediate Time Scale Motions	Journal of the American Chemical Society	2006
TAR-RNA recognition by a novel cyclic aminoglycoside analogue	Nucleic Acids Research	2006
Influence of the 2′-Hydroxyl Group Conformation on the Stability of A-form Helices in RNA	Journal of Molecular Biology	2006
A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin	Journal of Biomolecular NMR	2006
Side-Chain Orientation and Hydrogen-Bonding Imprint Supra-τc Motion on the Protein Backbone of Ubiquitin	Angewandte Chemie International Edition	2005
The INPHARMA Method: Protein-Mediated Interligand NOEs for Pharmacophore Mapping	Angewandte Chemie International Edition	2005
Ligand–Target Interactions: What Can We Learn from NMR?	Annual Review of Biophysics	2005
Identification of a Novel Pharmacophore for Peptide Toxins Interacting with K+ Channels	Journal of Biological Chemistry	2005
Assignment and NOE Analysis of 2'-Hydroxyl Protons in RNA: Implications for Stabilization of RNA A-Form Duplexes	Journal of the American Chemical Society	2005
κM-Conotoxin RIIIK, Structural and Functional Novelty in a K+ Channel Antagonist	Biochemistry	2004
Structure of the NCoA-1/SRC-1 PAS-B Domain Bound to the LXXLL Motif of the STAT6 Transactivation Domain	Journal of Molecular Biology	2004
Measuring the χ1 torsion angle in protein by CH-CH cross-correlated relaxation: A new resolution-optimised experiment	Journal of Biomolecular NMR	2003
Structural studies on a twin-arginine signal sequence	FEBS Letters	2003
The High-Resolution Solution Structure of Epothilone A Bound to Tubulin: An Understanding of the Structure–Activity Relationships for a Powerful Class of Antitumor Agents	Angewandte Chemie International Edition	2003
Derivation of Dihedral Angles from CH–CH Dipolar–Dipolar Cross-Correlated Relaxation Rates: A C–C Torsion Involving a Quaternary Carbon Atom in Epothilone A Bound to Tubulin	Angewandte Chemie International Edition	2003
A novel PH-CT-COSY methodology for measuring JPH coupling constants in unlabeled nucleic acids. Application to HIV-2 TAR RNA	Journal of Biomolecular NMR	2002
Determination of Aliphatic Side-Chain Conformation Using Cross-Correlated Relaxation: Application to an Extraordinarily Stable 2′-aminoethoxy-Modified Oligonucleotide Triplex	Journal of the American Chemical Society	2001
Residual Dipolar Coupling TOCSY for Direct Through Space Correlations of Base Protons and Phosphorus Nuclei in RNA	Journal of the American Chemical Society	2001
COIN TACSY, a Novel Approach to Tailored Correlation Spectroscopy	Journal of Magnetic Resonance	2001
Cross-Correlated Relaxation for Measurement of Angles between Tensorial Interactions	Methods in Enzymology	2001
A new method for the simultaneous measurement of magnitude and sign of 1DCH and 1DHH dipolar couplings in methylene groups	Journal of Biomolecular NMR	2000
Errors in the Measurement of Cross-Correlated Relaxation Rates and How to Avoid Them	Journal of Magnetic Resonance	2000
Ubiquitin Backbone Motion Studied via NHN−C‘Cα Dipolar−Dipolar and C‘−C‘Cα/NHN CSA−Dipolar Cross-Correlated Relaxation	Journal of the American Chemical Society	2000
Transferred Cross-Correlated Relaxation: Application to the Determination of Sugar Pucker in an Aminoacylated tRNA-Mimetic Weakly Bound to EF-Tu	Journal of the American Chemical Society	1999
New Methylene Specific Experiments for the Measurement of Scalar Spin–Spin Coupling Constants between Protons Attached to 13C	Journal of Magnetic Resonance	1998
“Kin” HEHAHA Sequences, Heteronuclear Hartmann–Hahn Transfer with Different Bandwidths for Spins I and S	Journal of Magnetic Resonance	1997
Resonance assignment and secondary structure determination and stability of the recombinant human uteroglobin with heteronuclear multidimensional NMR	Journal of Biomolecular NMR	1997
PLUSH TACSY: Homonuclear planar TACSY with two-band selective shaped pulses applied to Cα,C′ transfer and Cβ,Caromatic correlations	Journal of Biomolecular NMR	1996
Conformational analysis of Dolastatin 10: An NMR and theoretical approach	Biopolymers	1995