Prof. Teresa Carlomagno

Professor of Structural Biology

Academic Director of HWB·NMR

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About

Teresa Carlomagno started her scientific career at the University of Naples, Italy, where she studied Chemistry. During her Ph.D. studies and her following post-doctoral work at the University of Frankfurt, Germany, she developed NMR methods to study the structure and the dynamics of proteins, nucleic acids and small molecules. During a second post-doctoral period at The Scripps Research Institute, La Jolla, USA, she matured a strong interest in the mechanisms of intermolecular recognition and function in ribonucleoprotein complexes.

In 2001, she joined the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany, and started her own research group. In 2007, she moved to the EMBL in Heidelberg as a group leader in biomolecular NMR spectroscopy in the Structural and Computational Biology Unit. In 2015 she moved to the Leibniz University of Hannover, as a W3 professor in Structural Chemistry and joined group leader at the HZI in Braunschweig. In 2021 she left Germany to continue her career at the University of Birmingham, where she is Professor of Integrative Structural Biology at the School of Biosciences and in the Institute of Cancer and Genomics Sciences, as well as Academic Director of the national high-field NMR facility HWB-NMR.

Her laboratory characterizes intermolecular interactions at the border between structure, dynamics and disorder by combining NMR spectroscopy both in solution and in solid-state with XRS, EM, Small-Angle-Scattering (SAS) and Electron Paramagnetic Resonance (EPR). She uses integrative structural biology to study macromolecular complexes with enzymatic function involved in RNA metabolism and in the regulation of gene expression. In parallel, the lab develops the computational methodologies necessary to interpret and evaluate integrative structural biology data and new methodologies for structural biology of RNA. The lab has pioneered solid-state NMR as a tool to solve structures of RNA in flexible RNP complexes, which are not suitable for XRS and EM studies.

Awards & Honours
  • Member of the DFG Committee on Scientific Instrumentation and Information Technology 2017-2022

  • Chair of the ENC (conference with ca. 1000 participants) in Asilomar, California – April 19-24, 2015

  • Chair of the EMBO/EMBL Symposium “Molecular Machines: Lessons from integrating structure, biophysics and chemistry” – Heidelberg, Germany – May 18-21, 2014

  • Richard Ernst Stipendium from the Group of Magnetic Resonance of the GdCH (Gesellschaft Deutscher Chemiker, German Chemical Society) 1999

  • Alexander von Humboldt fellowship, 1997 (award declined in favor of the Marie Curie fellowship)

  • European Union grant of the „Marie Curie Researcher Training and Mobility Program“, 1997

  • Grant from the Italian Ministry of University, 1995

  • EMBO (European Molecular Biology Organization) fellowship, 1995

Publications within the group:


2021
Identification of RNA Base Pairs and Complete Assignment of Nucleobase Resonances by Proton-Detected Solid-State NMR Spectroscopy at 100 kHz MAS

Angewandte Chemie International Edition

1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein)

Biomolecular NMR Assignments

Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites

Scientific Reports

Recruitment of phospholipase Cγ1 to the non-structural membrane protein pK15 of Kaposi Sarcoma-associated herpesvirus promotes its Src-dependent phosphorylation

PLOS Pathogens

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Frontiers in Molecular Biosciences

Phosphotyrosine couples peptide binding and SHP2 activation via a dynamic allosteric network

Computational and Structural Biology Journal

High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain

RNA


2020
Structural and functional roles of 2’-O-ribose methylations and their enzymatic machinery across multiple classes of RNAs

Current Opinion in Structural Biology

1H, 13C, 15N chemical shift assignments of SHP2 SH2 domains in complex with PD-1 immune-tyrosine motifs

Biomolecular NMR Assignments

Observing Protein Degradation by the PAN-20S Proteasome by Time-Resolved Neutron Scattering

Biophysical Journal

Specificity and regulation of phosphotyrosine signaling through SH2 domains

Journal of Structural Biology: X

Structure of a Protein–RNA Complex by Solid-State NMR Spectroscopy

Angewandte Chemie International Edition

The guide sRNA sequence determines the activity level of box C/D RNPs

eLife

Small-Angle Neutron Scattering of RNA–Protein Complexes

Methods in Molecular Biology

Molecular mechanism of SHP2 activation by PD-1 stimulation

Science Advances


2019
Histone chaperone exploits intrinsic disorder to switch acetylation specificity

Nature Communications

Solid-state NMR Spectroscopy of RNA

Methods in Enzymology


2018
Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Chemical Communications

Isotope labeling for studying RNA by solid-state NMR spectroscopy

Journal of Biomolecular NMR

Structural characterization of the Asf1–Rtt109 interaction and its role in histone acetylation

Nucleic Acids Research


2017
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth

Origins Of Life And Evolution Of Biospheres

M3: an integrative framework for structure determination of molecular machines

Nature Methods

Structure-Based Design of Scaffolds Targeting PDE10A by INPHARMA-NMR

Journal of Chemical Information and Modeling


2016
A bromodomain–DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT

Nature Communications

The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats

Nucleic Acids Research

Optimization of protein samples for NMR using thermal shift assays

Journal of Biomolecular NMR


2015
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth Part I: Chemical Diversity, Oxygen and Nitrogen Based Polymers

Origins Of Life And Evolution Of Biospheres

A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin

Structure

Identification of new hit scaffolds by INPHARMA-guided virtual screening

MedChemComm

RNA structure determination by solid-state NMR spectroscopy

Nature Communications


2014
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

Nucleic Acids Research

Six Subgroups and Extensive Recent Duplications Characterize the Evolution of the Eukaryotic Tubulin Protein Family

Genome Biology and Evolution

Molecular Recognition of Epothilones by Microtubules and Tubulin Dimers Revealed by Biochemical and NMR Approaches

ACS Chemical Biology

Present and future of NMR for RNA–protein complexes: A perspective of integrated structural biology

Journal of Magnetic Resonance


2013
The structure of the box C/D enzyme reveals regulation of RNA methylation

Nature

A Reducing Milieu Renders Cofilin Insensitive to Phosphatidylinositol 4,5-Bisphosphate (PIP2) Inhibition

Journal of Biological Chemistry

A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex

Angewandte Chemie International Edition

Accounting for Conformational Variability in Protein–Ligand Docking with NMR-Guided Rescoring

Journal of the American Chemical Society

Structural Principles of RNA Catalysis in a 2′–5′ Lariat-Forming Ribozyme

Journal of the American Chemical Society

Towards Improved Quality of GPCR Models by Usage of Multiple Templates and Profile-Profile Comparison

PLOS ONE

Protein–RNA Interfaces Probed by 1H-Detected MAS Solid-State NMR Spectroscopy

Angewandte Chemie International Edition


2012
Synthesis and biological evaluation of a water-soluble derivative of the potent V-ATPase inhibitor archazolid

Bioorganic & Medicinal Chemistry Letters

Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity

Nature Structural & Molecular Biology

The description of protein internal motions aids selection of ligand binding poses by the INPHARMA method

Journal of Biomolecular NMR

Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding

Angewandte Chemie International Edition

NMR in natural products: understanding conformation, configuration and receptor interactions

Natural Product Reports

An NMR-based scoring function improves the accuracy of binding pose predictions by docking by two orders of magnitude

Journal of Biomolecular NMR


2008
Computational techniques are valuable tools for the discovery of protein–protein interaction inhibitors: The 14-3-3σ case

Bioorganic & Medicinal Chemistry Letters

NMR assignments of HIV-2 TAR RNA

Biomolecular NMR Assignments

Crystallography-Independent Determination of Ligand Binding Modes

Angewandte Chemie International Edition

Specific Methyl Group Protonation for the Measurement of Pharmacophore-Specific Interligand NOE Interactions

Chemistry: A European Journal

Studies of dynamic nuclear polarization with nitroxides in aqueous solution

Applied Magnetic Resonance

Construction of a liquid-state NMR DNP shuttle spectrometer: First experimental results and evaluation of optimal performance characteristics

Applied Magnetic Resonance

Argyrin A Reveals a Critical Role for the Tumor Suppressor Protein p27kip1 in Mediating Antitumor Activities in Response to Proteasome Inhibition

Cancer Cell

Simultaneous Determination of the Conformation and Relative Configuration of Archazolide A by Using Nuclear Overhauser Effects, J Couplings, and Residual Dipolar Couplings

Angewandte Chemie International Edition

Field dependent dynamic nuclear polarization with radicals in aqueous solution

Journal of the American Chemical Society

Conformational preferences of natural and C3-modified epothilones in aqueous solution

Journal of Medicinal Chemistry


2011
Recognition of 2′-O-Methylated 3′-End of piRNA by the PAZ Domain of a Piwi Protein

Structure


2010
Interaction of Epothilone B (Patupilone) with Microtubules as Detected by Two-Dimensional Solid-State NMR Spectroscopy

Angewandte Chemie International Edition

Structure of the K-turn U4 RNA: a combined NMR and SANS study

Nucleic Acids Research

Peptide Ligands Selected with CD4-Induced Epitopes on Native Dualtropic HIV-1 Envelope Proteins Mimic Extracellular Coreceptor Domains and Bind to HIV-1 gp120 Independently of Coreceptor Usage

Journal of Virology

The Tubulin-Bound Structure of the Antimitotic Drug Tubulysin

Angewandte Chemie International Edition

Synthesis and Biological Characterization of Argyrin F

ChemMedChem

The Binding Mode of Side Chain- and C3-Modified Epothilones to Tubulin

ChemMedChem

Elucidation of the Structure and Intermolecular Interactions of a Reversible Cyclic-Peptide Inhibitor of the Proteasome by NMR Spectroscopy and Molecular Modeling

Angewandte Chemie International Edition

1H, 13C and 15N resonance assignments of the Calmodulin-Munc13-1 peptide complex

Biomolecular NMR Assignments

Intermolecular Protein−RNA Interactions Revealed by 2D 31P−15N Magic Angle Spinning Solid-State NMR Spectroscopy

Journal Of The American Chemical Society

Drug Design for G-Protein-Coupled Receptors by a Ligand-Based NMR Method

Angewandte Chemie International Edition

Modular architecture of Munc13/calmodulin complexes: dual regulation by Ca2+ and possible function in short-term synaptic plasticity

EMBO Journal


2009
The INPHARMA technique for pharmacophore mapping: A theoretical guide to the method

Journal of Magnetic Resonance

An Efficient Strategy for the Determination of the Three-Dimensional Architecture of Ribonucleoprotein Complexes by the Combination of a Few Easily Accessible NMR and Biochemical Data: Intermolecular Recognition in a U4 Spliceosomal Complex

Journal of Molecular Biology

Probing Mutation-Induced Structural Perturbations by Refinement Against Residual Dipolar Couplings: Application to the U4 Spliceosomal RNP Complex

ChemBioChem


2007
13C-Detection in RNA Bases:  Revealing Structure−Chemical Shift Relationships

Journal of the American Chemical Society

Binding of the Human Prp31 Nop Domain to a Composite RNA–Protein Platform in U4 snRNP

Science

High-Resolution Solid-State NMR Structure of an Anticancer Agent

ChemMedChem

Structural Basis of the Activity of the Microtubule-Stabilizing Agent Epothilone A Studied by NMR Spectroscopy in Solution

Angewandte Chemie International Edition


2006
The Tubulin-Bound Conformation of Discodermolide Derived by NMR Studies in Solution Supports a Common Pharmacophore Model for Epothilone and Discodermolide

Angewandte Chemie International Edition

Calculation of the Dependence of Homo- and Heteronuclear 3J and 2J Scalar Couplings for the Determination of the 2′-Hydroxy Conformation in RNA

Angewandte Chemie International Edition

Stereochemical Determination of Archazolid A and B, Highly Potent Vacuolar-Type ATPase Inhibitors from the Myxobacterium Archangium gephyra

Organic Letters

SHARP-TACSY:  Triple-Band Tailored Correlated Spectroscopy for Base-to-Sugar Transfer in Nucleic Acid Residues with Intermediate Time Scale Motions

Journal of the American Chemical Society

TAR-RNA recognition by a novel cyclic aminoglycoside analogue

Nucleic Acids Research

Influence of the 2′-Hydroxyl Group Conformation on the Stability of A-form Helices in RNA

Journal of Molecular Biology

A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin

Journal of Biomolecular NMR


2005
Side-Chain Orientation and Hydrogen-Bonding Imprint Supra-τc Motion on the Protein Backbone of Ubiquitin

Angewandte Chemie International Edition

The INPHARMA Method: Protein-Mediated Interligand NOEs for Pharmacophore Mapping

Angewandte Chemie International Edition

Ligand-Target Interactions: What Can We Learn from NMR?

Annual Review of Biophysics and Biomolecular Structure

Identification of a Novel Pharmacophore for Peptide Toxins Interacting with K+ Channels

Journal of Biological Chemistry

Assignment and NOE Analysis of 2'-Hydroxyl Protons in RNA:  Implications for Stabilization of RNA A-Form Duplexes

Journal of the American Chemical Society


2004
κM-Conotoxin RIIIK, Structural and Functional Novelty in a K+ Channel Antagonist

Biochemistry

Structure of the NCoA-1/SRC-1 PAS-B Domain Bound to the LXXLL Motif of the STAT6 Transactivation Domain

Journal of Molecular Biology


2003
Measuring the χ1 torsion angle in protein by CH-CH cross-correlated relaxation: A new resolution-optimised experiment

Journal Of Biomolecular NMR

Structural studies on a twin-arginine signal sequence

FEBS Letters

The High-Resolution Solution Structure of Epothilone A Bound to Tubulin: An Understanding of the Structure–Activity Relationships for a Powerful Class of Antitumor Agents

Angewandte Chemie International Edition

Derivation of Dihedral Angles from CH–CH Dipolar–Dipolar Cross-Correlated Relaxation Rates: A C–C Torsion Involving a Quaternary Carbon Atom in Epothilone A Bound to Tubulin

Angewandte Chemie International Edition


2002
A novel PH-CT-COSY methodology for measuring JPH coupling constants in unlabeled nucleic acids. Application to HIV-2 TAR RNA

Journal of Biomolecular NMR


2001
Determination of Aliphatic Side-Chain Conformation Using Cross-Correlated Relaxation:  Application to an Extraordinarily Stable 2‘-aminoethoxy-Modified Oligonucleotide Triplex

Journal of the American Chemical Society

Residual Dipolar Coupling TOCSY for Direct Through Space Correlations of Base Protons and Phosphorus Nuclei in RNA

Journal of the American Chemical Society

COIN TACSY, a Novel Approach to Tailored Correlation Spectroscopy

Journal of Magnetic Resonance

Cross-Correlated Relaxation for Measurement of Angles between Tensorial Interactions

Methods in Enzymology


2000
A new method for the simultaneous measurement of magnitude and sign of 1DCH and 1DHH dipolar couplings in methylene groups

Journal of Biomolecular NMR

Errors in the Measurement of Cross-Correlated Relaxation Rates and How to Avoid Them

Journal of Magnetic Resonance

Ubiquitin Backbone Motion Studied via NHN−C‘Cα Dipolar−Dipolar and C‘−C‘Cα/NHN CSA−Dipolar Cross-Correlated Relaxation

Journal of the American Chemical Society


1999
Transferred Cross-Correlated Relaxation: Application to the Determination of Sugar Pucker in an Aminoacylated tRNA-Mimetic Weakly Bound to EF-Tu

Journal of the American Chemical Society


1998
New Methylene Specific Experiments for the Measurement of Scalar Spin–Spin Coupling Constants between Protons Attached to 13C

Journal of Magnetic Resonance


1997
“Kin” HEHAHA Sequences, Heteronuclear Hartmann–Hahn Transfer with Different Bandwidths for Spins I and S

Journal of Magnetic Resonance

Resonance assignment and secondary structure determination and stability of the recombinant human uteroglobin with heteronuclear multidimensional NMR

Journal of Biomolecular NMR


1996
PLUSH TACSY: Homonuclear planar TACSY with two-band selective shaped pulses applied to Cα,C′ transfer and Cβ,Caromatic correlations

Journal of Biomolecular NMR


1995
Conformational analysis of Dolastatin 10: An NMR and theoretical approach

Biopolymers