Prof. Teresa Carlomagno

Academic Director of Biomolecular NMR

Professor of Structural Biology

About

Teresa Carlomagno started her scientific career at the University of Naples, Italy, where she studied Chemistry. During her Ph.D. studies and her following post-doctoral work at the University of Frankfurt, Germany, she developed NMR methods to study the structure and the dynamics of proteins, nucleic acids and small molecules. During a second post-doctoral period at The Scripps Research Institute, La Jolla, USA, she matured a strong interest in the mechanisms of intermolecular recognition and function in ribonucleoprotein complexes.

In 2001, she joined the Max Planck Institute for Biophysical Chemistry in Gƶttingen, Germany, and started her own research group. In 2007, she moved to the EMBL in Heidelberg as a group leader in biomolecular NMR spectroscopy in the Structural and Computational Biology Unit. In 2015 she moved to the Leibniz University of Hannover, as a W3 professor in Structural Chemistry and joined group leader at the HZI in Braunschweig. In 2021 she left Germany to continue her career at the University of Birmingham, where she is Professor of Integrative Structural Biology at the School of Biosciences and in the Institute of Cancer and Genomics Sciences, as well as Academic Director of the national high-field NMR facility HWB-NMR.

Her laboratory characterizes intermolecular interactions at the border between structure, dynamics and disorder by combining NMR spectroscopy both in solution and in solid-state with XRS, EM, Small-Angle-Scattering (SAS) and Electron Paramagnetic Resonance (EPR). She uses integrative structural biology to study macromolecular complexes with enzymatic function involved in RNA metabolism and in the regulation of gene expression. In parallel, the lab develops the computational methodologies necessary to interpret and evaluate integrative structural biology data and new methodologies for structural biology of RNA. The lab has pioneered solid-state NMR as a tool to solve structures of RNA in flexible RNP complexes, which are not suitable for XRS and EM studies.

Awards and Honours:
  • Member of the DFG Committee on Scientific Instrumentation and Information Technology 2017ā€“2022
  • Chair of the ENC (conference with ca. 1000 participants) in Asilomar, California ā€“ April 19ā€“24, 2015
  • Chair of the EMBO/EMBL Symposium "Molecular Machines: Lessons from integrating structure, biophysics and chemistry" ā€“ Heidelberg, Germany ā€“ May 18ā€“21, 2014
  • Richard Ernst Stipendium from the Group of Magnetic Resonance of the GdCH (Gesellschaft Deutscher Chemiker, German Chemical Society) 1999
  • Alexander von Humboldt fellowship, 1997 (award declined in favor of the Marie Curie fellowship)
  • European Union grant of the "Marie Curie Researcher Training and Mobility Program", 1997
  • Grant from the Italian Ministry of University, 1995
  • EMBO (European Molecular Biology Organization) fellowship, 1995

Publications within the group:

Long-range conformational changes in the nucleotide-bound states of the DEAD-box helicase Vasa Biophysical Journal 2024
The specificity of intermodular recognition in a prototypical nonribosomal peptide synthetase depends on an adaptor domain Science Advances 2024
Phosphopeptide binding to the N-SH2 domain of tyrosine phosphatase SHP2 correlates with the unzipping of its central Ī²-sheet Computational and Structural Biology Journal 2024
Methods for Structure Determination of SH2 Domainā€“Phosphopeptide Complexes by NMR Methods in Molecular Biology 2023
NMR Methods to Study the Dynamics of SH2 Domainā€“Phosphopeptide Complexes Methods in Molecular Biology 2023
An NMR Study of a 300-kDa AAA+ Unfoldase Journal of Molecular Biology 2023
Experiment-guided molecular simulations define a heterogeneous structural ensemble for the PTPN11 tandem SH2 domains Chemical Science 2023
A real-time analysis of GFP unfolding by the AAA+ unfoldase PAN Journal of Magnetic Resonance 2023
The EJC disassembly factor PYM is an intrinsically disordered protein and forms a fuzzy complex with RNA Frontiers in Molecular Biosciences 2023
Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2 Structure 2023
Nucleic acidā€“protein interfaces studied by MAS solid-state NMR spectroscopy Journal of Structural Biology: X 2022
Identification of RNA Base Pairs and Complete Assignment of Nucleobase Resonances by Proton-Detected Solid-State NMR Spectroscopy at 100ā€…kHz MAS Angewandte Chemie International Edition 2021
1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein) Biomolecular NMR Assignments 2021
Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites Scientific Reports 2021
Recruitment of phospholipase CĪ³1 to the non-structural membrane protein pK15 of Kaposi Sarcoma-associated herpesvirus promotes its Src-dependent phosphorylation PLOS Pathogens 2021
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications Frontiers in Molecular Biosciences 2021
Phosphotyrosine couples peptide binding and SHP2 activation via a dynamic allosteric network Computational and Structural Biology Journal 2021
High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain RNA 2021
Structural and functional roles of 2ā€²-O-ribose methylations and their enzymatic machinery across multiple classes of RNAs Current Opinion in Structural Biology 2020
1H, 13C, 15N chemical shift assignments of SHP2 SH2 domains in complex with PD-1 immune-tyrosine motifs Biomolecular NMR Assignments 2020
Observing Protein Degradation by the PAN-20S Proteasome by Time-Resolved Neutron Scattering Biophysical Journal 2020
Specificity and regulation of phosphotyrosine signaling through SH2 domains Journal of Structural Biology: X 2020
Structure of a Proteinā€“RNA Complex by Solid-State NMR Spectroscopy Angewandte Chemie International Edition 2020
The guide sRNA sequence determines the activity level of box C/D RNPs eLife 2020
Small-Angle Neutron Scattering of RNAā€“Protein Complexes Methods in Molecular Biology 2020
Molecular mechanism of SHP2 activation by PD-1 stimulation Science Advances 2020
Molecular mechanism of SHP2 activation by PD-1 stimulation Science Advances 2020
Histone chaperone exploits intrinsic disorder to switch acetylation specificity Nature Communications 2019
Solid-state NMR Spectroscopy of RNA Methods in Enzymology 2018
Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS Chemical Communications 2018
Isotope labeling for studying RNA by solid-state NMR spectroscopy Journal of Biomolecular NMR 2018
Structural characterization of the Asf1ā€“Rtt109 interaction and its role in histone acetylation Nucleic Acids Research 2018
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth Origins of Life and Evolution of Biospheres 2017
M3: an integrative framework for structure determination of molecular machines Nature Methods 2017
Structure-Based Design of Scaffolds Targeting PDE10A by INPHARMA-NMR Journal of Chemical Information and Modeling 2017
A bromodomainā€“DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT Nature Communications 2016
RflM mediates target specificity of the RcsCDB phosphorelay system for transcriptional repression of flagellar synthesis in Salmonella enterica Molecular Microbiology 2016
Chemical Analysis of a "Miller-Type" Complex Prebiotic Broth Part I: Chemical Diversity, Oxygen and Nitrogen Based Polymers Origins of Life and Evolution of Biospheres 2016
Archaea box C/D enzymes methylate two distinct substrate rRNA sequences with different efficiency RNA 2016
The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats Nucleic Acids Research 2016
Optimization of protein samples for NMR using thermal shift assays Journal of Biomolecular NMR 2016
On-the-Fly Integration of Data from a Spin-Diffusion-Based NMR Experiment into Proteinā€“Ligand Docking Journal of Chemical Information and Modeling 2015
A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin Structure 2015
The structure of the SOLE element of oskar mRNA RNA 2015
Identification of new hit scaffolds by INPHARMA-guided virtual screening MedChemComm 2015
RNA structure determination by solid-state NMR spectroscopy Nature Communications 2015
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum Nucleic Acids Research 2014
Six Subgroups and Extensive Recent Duplications Characterize the Evolution of the Eukaryotic Tubulin Protein Family Genome Biology and Evolution 2014
Molecular Recognition of Epothilones by Microtubules and Tubulin Dimers Revealed by Biochemical and NMR Approaches ACS Chemical Biology 2014
Present and future of NMR for RNAā€“protein complexes: A perspective of integrated structural biology Journal of Magnetic Resonance 2014
The structure of the box C/D enzyme reveals regulation of RNA methylation Nature 2013
A Reducing Milieu Renders Cofilin Insensitive to Phosphatidylinositol 4,5-Bisphosphate (PIP2) Inhibition Journal of Biological Chemistry 2013
A Suite of Solid-State NMR Experiments for RNA Intranucleotide Resonance Assignment in a 21 kDa Protein-RNA Complex Angewandte Chemie International Edition 2013
Accounting for Conformational Variability in Proteinā€“Ligand Docking with NMR-Guided Rescoring Journal of the American Chemical Society 2013
Structural Principles of RNA Catalysis in a 2ā€²ā€“5ā€² Lariat-Forming Ribozyme Journal of the American Chemical Society 2013
Towards Improved Quality of GPCR Models by Usage of Multiple Templates and Profile-Profile Comparison PLOS ONE 2013
Proteinā€“RNA Interfaces Probed by 1H-Detected MAS Solid-State NMR Spectroscopy Angewandte Chemie International Edition 2013
Synthesis and biological evaluation of a water-soluble derivative of the potent V-ATPase inhibitor archazolid Bioorganic & Medicinal Chemistry Letters 2012
Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity Nature Structural and Molecular Biology 2012
The description of protein internal motions aids selection of ligand binding poses by the INPHARMA method Journal of Biomolecular NMR 2012
Understanding the Inhibitory Effect of Highly Potent and Selective Archazolides Binding to the Vacuolar ATPase Journal of Chemical Information and Modeling 2012
Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding Angewandte Chemie International Edition 2012
NMR in natural products: understanding conformation, configuration and receptor interactions Natural Product Reports 2012
An NMR-based scoring function improves the accuracy of binding pose predictions by docking by two orders of magnitude Journal of Biomolecular NMR 2012
Computational techniques are valuable tools for the discovery of proteinā€“protein interaction inhibitors: The 14-3-3Ļƒ case Bioorganic & Medicinal Chemistry Letters 2011
Recognition of 2ā€²-O-Methylated 3ā€²-End of piRNA by the PAZ Domain of a Piwi Protein Structure 2011
Interaction of Epothilone B (Patupilone) with Microtubules as Detected by Two-Dimensional Solid-State NMR Spectroscopy Angewandte Chemie International Edition 2010
Structure of the K-turn U4 RNA: a combined NMR and SANS study Nucleic Acids Research 2010
Peptide Ligands Selected with CD4-Induced Epitopes on Native Dualtropic HIV-1 Envelope Proteins Mimic Extracellular Coreceptor Domains and Bind to HIV-1 gp120 Independently of Coreceptor Usage Journal of Virology 2010
The Tubulin-Bound Structure of the Antimitotic Drug Tubulysin Angewandte Chemie International Edition 2010
Synthesis and Biological Characterization of Argyrinā€…F ChemMedChem 2010
The Binding Mode of Side Chain- and C3-Modified Epothilones to Tubulin ChemMedChem 2010
Elucidation of the Structure and Intermolecular Interactions of a Reversible Cyclic-Peptide Inhibitor of the Proteasome by NMR Spectroscopy and Molecular Modeling Angewandte Chemie International Edition 2010
1H, 13C and 15N resonance assignments of the Calmodulin-Munc13-1 peptide complex Biomolecular NMR Assignments 2010
Intermolecular Proteināˆ’RNA Interactions Revealed by 2D 31Pāˆ’15N Magic Angle Spinning Solid-State NMR Spectroscopy Journal of the American Chemical Society 2010
Drug Design for G-Protein-Coupled Receptors by a Ligand-Based NMR Method Angewandte Chemie International Edition 2010
Modular architecture of Munc13/calmodulin complexes: dual regulation by Ca2+ and possible function in short-term synaptic plasticity EMBO Journal 2010
The INPHARMA technique for pharmacophore mapping: A theoretical guide to the method Journal of Magnetic Resonance 2009
An Efficient Strategy for the Determination of the Three-Dimensional Architecture of Ribonucleoprotein Complexes by the Combination of a Few Easily Accessible NMR and Biochemical Data: Intermolecular Recognition in a U4 Spliceosomal Complex Journal of Molecular Biology 2009
Probing Mutation-Induced Structural Perturbations by Refinement Against Residual Dipolar Couplings: Application to the U4 Spliceosomal RNP Complex ChemBioChem 2009
NMR assignments of HIV-2 TAR RNA Biomolecular NMR Assignments 2008
Crystallography-Independent Determination of Ligand Binding Modes Angewandte Chemie International Edition 2008
Specific Methyl Group Protonation for the Measurement of Pharmacophore-Specific Interligand NOE Interactions Chemistry: A European Journal 2008
Studies of dynamic nuclear polarization with nitroxides in aqueous solution Applied Magnetic Resonance 2008
Construction of a liquid-state NMR DNP shuttle spectrometer: First experimental results and evaluation of optimal performance characteristics Applied Magnetic Resonance 2008
Argyrin A Reveals a Critical Role for the Tumor Suppressor Protein p27kip1 in Mediating Antitumor Activities in Response to Proteasome Inhibition Cancer Cell 2008
Simultaneous Determination of the Conformation and Relative Configuration of Archazolideā€…A by Using Nuclear Overhauser Effects, J Couplings, and Residual Dipolar Couplings Angewandte Chemie International Edition 2008
Field dependent dynamic nuclear polarization with radicals in aqueous solution Journal of the American Chemical Society 2008
Conformational preferences of natural and C3-modified epothilones in aqueous solution Journal of Medicinal Chemistry 2008
13C-Detection in RNA Bases:ā€‰ Revealing Structureāˆ’Chemical Shift Relationships Journal of the American Chemical Society 2007
Binding of the Human Prp31 Nop Domain to a Composite RNAā€“Protein Platform in U4 snRNP Science 2007
High-Resolution Solid-State NMR Structure of an Anticancer Agent ChemMedChem 2007
Structural Basis of the Activity of the Microtubule-Stabilizing Agent Epothiloneā€…A Studied by NMR Spectroscopy in Solution Angewandte Chemie International Edition 2007
The Tubulin-Bound Conformation of Discodermolide Derived by NMR Studies in Solution Supports a Common Pharmacophore Model for Epothilone and Discodermolide Angewandte Chemie International Edition 2006
Calculation of the Dependence of Homo- and Heteronuclear 3J and 2J Scalar Couplings for the Determination of the 2ā€²-Hydroxy Conformation in RNA Angewandte Chemie International Edition 2006
Stereochemical Determination of Archazolid A and B, Highly Potent Vacuolar-Type ATPase Inhibitors from the Myxobacterium Archangium gephyra Organic Letters 2006
SHARP-TACSY:ā€‰ Triple-Band Tailored Correlated Spectroscopy for Base-to-Sugar Transfer in Nucleic Acid Residues with Intermediate Time Scale Motions Journal of the American Chemical Society 2006
TAR-RNA recognition by a novel cyclic aminoglycoside analogue Nucleic Acids Research 2006
Influence of the 2ā€²-Hydroxyl Group Conformation on the Stability of A-form Helices in RNA Journal of Molecular Biology 2006
A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin Journal of Biomolecular NMR 2006
Side-Chain Orientation and Hydrogen-Bonding Imprint Supra-Ļ„c Motion on the Protein Backbone of Ubiquitin Angewandte Chemie International Edition 2005
The INPHARMA Method: Protein-Mediated Interligand NOEs for Pharmacophore Mapping Angewandte Chemie International Edition 2005
Ligandā€“Target Interactions: What Can We Learn from NMR? Annual Review of Biophysics 2005
Identification of a Novel Pharmacophore for Peptide Toxins Interacting with K+ Channels Journal of Biological Chemistry 2005
Assignment and NOE Analysis of 2'-Hydroxyl Protons in RNA:ā€‰ Implications for Stabilization of RNA A-Form Duplexes Journal of the American Chemical Society 2005
ĪŗM-Conotoxin RIIIK, Structural and Functional Novelty in a K+ Channel Antagonist Biochemistry 2004
Structure of the NCoA-1/SRC-1 PAS-B Domain Bound to the LXXLL Motif of the STAT6 Transactivation Domain Journal of Molecular Biology 2004
Measuring the Ļ‡1 torsion angle in protein by CH-CH cross-correlated relaxation: A new resolution-optimised experiment Journal of Biomolecular NMR 2003
Structural studies on a twin-arginine signal sequence FEBS Letters 2003
The High-Resolution Solution Structure of Epothilone A Bound to Tubulin: An Understanding of the Structureā€“Activity Relationships for a Powerful Class of Antitumor Agents Angewandte Chemie International Edition 2003
Derivation of Dihedral Angles from CHā€“CH Dipolarā€“Dipolar Cross-Correlated Relaxation Rates: A Cā€“C Torsion Involving a Quaternary Carbon Atom in Epothilone A Bound to Tubulin Angewandte Chemie International Edition 2003
A novel PH-CT-COSY methodology for measuring JPH coupling constants in unlabeled nucleic acids. Application to HIV-2 TAR RNA Journal of Biomolecular NMR 2002
Determination of Aliphatic Side-Chain Conformation Using Cross-Correlated Relaxation:ā€‰ Application to an Extraordinarily Stable 2ā€²-aminoethoxy-Modified Oligonucleotide Triplex Journal of the American Chemical Society 2001
Residual Dipolar Coupling TOCSY for Direct Through Space Correlations of Base Protons and Phosphorus Nuclei in RNA Journal of the American Chemical Society 2001
COIN TACSY, a Novel Approach to Tailored Correlation Spectroscopy Journal of Magnetic Resonance 2001
Cross-Correlated Relaxation for Measurement of Angles between Tensorial Interactions Methods in Enzymology 2001
A new method for the simultaneous measurement of magnitude and sign of 1DCH and 1DHH dipolar couplings in methylene groups Journal of Biomolecular NMR 2000
Errors in the Measurement of Cross-Correlated Relaxation Rates and How to Avoid Them Journal of Magnetic Resonance 2000
Ubiquitin Backbone Motion Studied via NHNāˆ’Cā€˜CĪ± Dipolarāˆ’Dipolar and Cā€˜āˆ’Cā€˜CĪ±/NHN CSAāˆ’Dipolar Cross-Correlated Relaxation Journal of the American Chemical Society 2000
Transferredā€‰Cross-Correlatedā€‰Relaxation:ā€‰Application to the Determination of Sugar Pucker in an Aminoacylated tRNA-Mimetic Weakly Bound to EF-Tu Journal of the American Chemical Society 1999
New Methylene Specific Experiments for the Measurement of Scalar Spinā€“Spin Coupling Constants between Protons Attached to 13C Journal of Magnetic Resonance 1998
ā€œKinā€ HEHAHA Sequences, Heteronuclear Hartmannā€“Hahn Transfer with Different Bandwidths for Spins I and S Journal of Magnetic Resonance 1997
Resonance assignment and secondary structure determination and stability of the recombinant human uteroglobin with heteronuclear multidimensional NMR Journal of Biomolecular NMR 1997
PLUSH TACSY: Homonuclear planar TACSY with two-band selective shaped pulses applied to CĪ±,Cā€² transfer and CĪ²,Caromatic correlations Journal of Biomolecular NMR 1996
Conformational analysis of Dolastatin 10: An NMR and theoretical approach Biopolymers 1995