Dr John Kirkpatrick

Joined the group

Publications within the group:


2024
The specificity of intermodular recognition in a prototypical nonribosomal peptide synthetase depends on an adaptor domain

Science Advances

Phosphopeptide binding to the N-SH2 domain of tyrosine phosphatase SHP2 correlates with the unzipping of its central β-sheet

Computational and Structural Biology Journal


2023
NMR Methods to Study the Dynamics of SH2 Domain-Phosphopeptide Complexes

Methods in Molecular Biology

Methods for Structure Determination of SH2 Domain-Phosphopeptide Complexes by NMR

Methods in Molecular Biology

An NMR Study of a 300-kDa AAA+ Unfoldase

Journal of Molecular Biology

Experiment-guided molecular simulations define a heterogeneous structural ensemble for the PTPN11 tandem SH2 domains

Chemical Science

A real-time analysis of GFP unfolding by the AAA+ unfoldase PAN

Journal of Magnetic Resonance

The EJC disassembly factor PYM is an intrinsically disordered protein and forms a fuzzy complex with RNA

Frontiers in Molecular Biosciences

Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2

Structure


2021
Identification of RNA Base Pairs and Complete Assignment of Nucleobase Resonances by Proton-Detected Solid-State NMR Spectroscopy at 100 kHz MAS

Angewandte Chemie International Edition

1H, 13C, and 15N backbone chemical-shift assignments of SARS-CoV-2 non-structural protein 1 (leader protein)

Biomolecular NMR Assignments

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Frontiers in Molecular Biosciences

Phosphotyrosine couples peptide binding and SHP2 activation via a dynamic allosteric network

Computational and Structural Biology Journal


2020
1H, 13C, 15N chemical shift assignments of SHP2 SH2 domains in complex with PD-1 immune-tyrosine motifs

Biomolecular NMR Assignments

The guide sRNA sequence determines the activity level of box C/D RNPs

eLife

Molecular mechanism of SHP2 activation by PD-1 stimulation

Science Advances


2019
Histone chaperone exploits intrinsic disorder to switch acetylation specificity

Nature Communications


2018
Structural characterization of the Asf1–Rtt109 interaction and its role in histone acetylation

Nucleic Acids Research


2011
Recognition of 2′-O-Methylated 3′-End of piRNA by the PAZ Domain of a Piwi Protein

Structure


2010
Intermolecular Protein−RNA Interactions Revealed by 2D 31P−15N Magic Angle Spinning Solid-State NMR Spectroscopy

Journal Of The American Chemical Society


2009
An Efficient Strategy for the Determination of the Three-Dimensional Architecture of Ribonucleoprotein Complexes by the Combination of a Few Easily Accessible NMR and Biochemical Data: Intermolecular Recognition in a U4 Spliceosomal Complex

Journal of Molecular Biology

Probing Mutation-Induced Structural Perturbations by Refinement Against Residual Dipolar Couplings: Application to the U4 Spliceosomal RNP Complex

ChemBioChem