I obtained my undergraduate degree in Biochemistry from the University of Oxford. During this time, I worked with Prof. Anthony Watts on the characterisation of membrane nanodiscs by biophysical and magnetic resonance techniques. For my thesis, I worked in Dr. Jason Schnell's lab on solution state NMR of membrane proteins. Since joining the Carlomagno group at EMBL Heidelberg in 2012, I have worked on characterising proteins and protein/RNA complexes by integrating NMR and solution scattering techniques, as well as other biochemical and biophysical methods. My main focus has been on elucidating the mechanism and regulation of the 400-KDa Box C/D snoRNP enzyme at the structural and biochemical levels.
Small-angle scattering techniques (X-Ray, Neutron)
Bacterial protein expression & purification, in vitro protein complex reconstitution
Glatt S, Zabel R, Kolaj-Robin O, Onuma OF, Baudin F, Graziadei A, Taverniti V, Lin TY, Baymann F, Seraphin B, Breunig KD, Muller CW
Structural basis for tRNA modification by Elp3 from Dehalococcoides mccartyi.
Nat Struct Mol Biol. 2016 Sep;23(9):794-802. doi: link
Graziadei A, Masiewicz P, Lapinaite A, Carlomagno T
Archaea box C/D enzymes methylate two distinct substrate rRNA sequences with different efficiency.
RNA. 2016 May;22(5):764-72. doi: link
Orwick-Rydmark M, Lovett JE, Graziadei A, Lindholm L, Hicks MR, Watts A
Detergent-free incorporation of a seven-transmembrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies.
Nano Lett. 2012 Sep 12;12(9):4687-92. doi: link
Orwick MC, Judge PJ, Procek J, Lindholm L, Graziadei A, Engel A, Grobner G, Watts A
Detergent-free formation and physicochemical characterization of nanosized lipid-polymer complexes: Lipodisq.
Angew Chem Int Ed Engl. 2012 May 7;51(19):4653-7. doi: link