Interaction of Epothilone B (Patupilone) with Microtubules as Detected by Two-Dimensional Solid-State NMR Spectroscopy

A Kumar, H Heise, MJJ Blommers, P Krastel, E Schmitt, F Petersen, S Jeganathan, EM Mandelkow, T Carlomagno, C Griesinger & M Baldus

Angewandte Chemie International Edition

Pages
7504–7507
Issue
41
Volume
49
Year
2010

Solid evidence: Induction of the polymerization of αβ-tubulin dimers into microtubules by epothilones, such as patupilone, by an as yet unknown mechanism leads to the apoptosis of cancer cells. Solid-state NMR spectroscopy of patupilone bound to microtubules has now enabled the identification of atomic positions of the drug that undergo clear chemical-shift changes upon binding (see correlation spectra of free (black) and complexed patupilone (red)).