Derivation of Dihedral Angles from CH–CH Dipolar–Dipolar Cross-Correlated Relaxation Rates: A C–C Torsion Involving a Quaternary Carbon Atom in Epothilone A Bound to Tubulin
Angewandte Chemie International Edition
Pages
2515–2517
Issue
22
Volume
42
Year
2003
C–C torsion angles in HC-CCH3 moieties can be measured by a new NMR experiment based on the measurement of CH–CH dipolar–dipolar cross-correlated relaxation rates and is demonstrated on a mixture of 13C-labeled epothilone (1) and tubulin. The new pulse sequence is broadly applicable to any HC-CCH3 moiety and therefore to a number of amino acid side chains in proteins.