John Kirkpatrick

Summary

Specialties:

Publications:

2015

Nyon MP, Prentice T, Day J, Kirkpatrick J, Sivalingam GN, Levy G, Haq I, Irving JA, Lomas DA, Christodoulou J, Gooptu B, Thalassinos K

An integrative approach combining ion mobility mass spectrometry, X-ray crystallography and NMR spectroscopy to study the conformational dynamics of alpha -antitrypsin upon ligand binding.

Protein Sci. 2015 May 26. doi: link


2014

Shiltagh N, Kirkpatrick J, Cabrita LD, McKinnon TA, Thalassinos K, Tuddenham EG, Hansen DF

Solution structure of the major factor VIII binding region on von Willebrand factor.

Blood. 2014 Jun 26;123(26):4143-51. doi: link


Werbeck ND, Kirkpatrick J, Reinstein J, Hansen DF

Using 15N-ammonium to characterise and map potassium binding sites in proteins by NMR spectroscopy.

Chembiochem. 2014 Mar 3;15(4):543-8. doi: link


Rasheed M, Richter C, Chisty LT, Kirkpatrick J, Blackledge M, Webb MR, Driscoll PC

Ligand-dependent dynamics of the active-site lid in bacterial dimethylarginine dimethylaminohydrolase.

Biochemistry. 2014 Feb 18;53(6):1092-104. doi: link


2013

Kunze MB, Wright DW, Werbeck ND, Kirkpatrick J, Coveney PV, Hansen DF

Loop interactions and dynamics tune the enzymatic activity of the human histone deacetylase 8.

J Am Chem Soc. 2013 Nov 27;135(47):17862-8. doi: link


Werbeck ND, Kirkpatrick J, Hansen DF

Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH.

Angew Chem Int Ed Engl. 2013 Mar 11;52(11):3145-7. doi: link


2012

Nyon MP, Segu L, Cabrita LD, Levy GR, Kirkpatrick J, Roussel BD, Patschull AO, Barrett TE, Ekeowa UI, Kerr R, Waudby CA, Kalsheker N, Hill M, Thalassinos K, Lomas DA, Christodoulou J, Gooptu B

Structural dynamics associated with intermediate formation in an archetypal conformational disease.

Structure. 2012 Mar 7;20(3):504-12. doi: link


Nyon MP, Kirkpatrick J, Cabrita LD, Christodoulou J, Gooptu B

1H, 15N and 13C backbone resonance assignments of the archetypal serpin alpha1-antitrypsin.

Biomol NMR Assign. 2012 Oct;6(2):153-6. doi: link


2011

Simon B, Kirkpatrick JP, Eckhardt S, Reuter M, Rocha EA, Andrade-Navarro MA, Sehr P, Pillai RS, Carlomagno T

Recognition of 2'-O-methylated 3'-end of piRNA by the PAZ domain of a Piwi protein.

Structure. 2011 Feb 9;19(2):172-80. doi: link


2010

Gautier A, Mott HR, Bostock MJ, Kirkpatrick JP, Nietlispach D

Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy.

Nat Struct Mol Biol. 2010 Jun;17(6):768-74. doi: link


Jansma AL, Kirkpatrick JP, Hsu AR, Handel TM, Nietlispach D

NMR analysis of the structure, dynamics, and unique oligomerization properties of the chemokine CCL27.

J Biol Chem. 2010 May 7;285(19):14424-37. doi: link


Jehle S, Falb M, Kirkpatrick JP, Oschkinat H, van Rossum BJ, Althoff G, Carlomagno T

Intermolecular protein-RNA interactions revealed by 2D 31P-15N magic angle spinning solid-state NMR spectroscopy.

J Am Chem Soc. 2010 Mar 24;132(11):3842-6. doi: link


2009

Kirkpatrick JP, Li P, Carlomagno T

Probing mutation-induced structural perturbations by refinement against residual dipolar couplings: application to the U4 spliceosomal RNP complex.

Chembiochem. 2009 Apr 17;10(6):1007-14. doi: link


Li P, Kirkpatrick J, Carlomagno T

An efficient strategy for the determination of the three-dimensional architecture of ribonucleoprotein complexes by the combination of a few easily accessible NMR and biochemical data: intermolecular recognition in a U4 spliceosomal complex.

J Mol Biol. 2009 May 1;388(2):283-98. doi: link


2008

Gautier A, Kirkpatrick JP, Nietlispach D

Solution-state NMR spectroscopy of a seven-helix transmembrane protein receptor: backbone assignment, secondary structure, and dynamics.

Angew Chem Int Ed Engl. 2008;47(38):7297-300. doi: link


2007

Allen SJ, Zabel BA, Kirkpatrick J, Butcher EC, Nietlispach D, Handel TM

NMR assignment of human chemerin, a novel chemoattractant.

Biomol NMR Assign. 2007 Dec;1(2):171-3. doi: link


2006

Bois PR, O'Hara BP, Nietlispach D, Kirkpatrick J, Izard T

The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin.

J Biol Chem. 2006 Mar 17;281(11):7228-36. Epub 2006 Jan 9.